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polystyrene 96 well round bottom plates  (Greiner Bio)


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    Greiner Bio polystyrene 96 well round bottom plates
    Polystyrene 96 Well Round Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polystyrene+96+well+round+bottom+plates/Microplate+96+Well+Ps+U-Bottom+Clear/us12595316-357-9-13
    Average 96 stars, based on 409 article reviews
    polystyrene 96 well round bottom plates - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Determination of Complement Dependent Cytotoxicity (CDC) In a first experiment, tumor cells derived from an untreated CLL patient (AllCells, California, USA), were resuspended in RPMI containing 0.2% BSA (bovine serum albumin) and plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.2×105 cells/well (40 μL/well) and 40 μL of a concentration series of IgG1-G28.1-K409R-delK, IgG1-G28.1-E345R or IgG1-b12-E345R (0.003-10 μg/mL final antibody concentration). ..

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: The capacity to induce CDC was tested for a combination of bsIgG1-016-H5L2-LC90S-F405L-E430G×010-H5L2-K409R-E430G and an anti-CD20 antibody (IgG1-CD20-ofa; ofatumumab) on patient derived CLL tumor cells obtained from ConversantBio (Huntsville, Alabama, USA). .. Patient derived PBMCs were resuspended in RPMI containing 0.2% BSA (bovine serum albumin) and plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.1×10 6 cells/well (30 μL/well) and 50 μL of a concentration series of bsIgG1-016-H5L2-LC90S-F405L-E430G×010-H5L2-K409R-E430G (0.0625-0.05 μg/mL) and IgG1-CD20-ofa (1-8 μg/mL) was added in 2-fold dilutions. ..

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Briefly, tumor cells derived from a CLL patient (as in first experiment described above), resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.5×105 cells/well (30 μL/well), centrifuged and 50 μL of CD37 (Abcam, cat. no. 76522) or control mouse antibody (Purified Mouse IgG1,κ Isotype Control, Clone MOPC-21; BD cat. no. 555746) was added. ..

    Concentration Assay:

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Determination of Complement Dependent Cytotoxicity (CDC) In a first experiment, tumor cells derived from an untreated CLL patient (AllCells, California, USA), were resuspended in RPMI containing 0.2% BSA (bovine serum albumin) and plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.2×105 cells/well (40 μL/well) and 40 μL of a concentration series of IgG1-G28.1-K409R-delK, IgG1-G28.1-E345R or IgG1-b12-E345R (0.003-10 μg/mL final antibody concentration). ..

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: Graphs were generated using best-fit values of a non-linear dose-response fit with log-transformed concentrations in GraphPad Prism V6.04 software (GraphPad Software, San Diego, CA, USA). .. In a second experiment, tumor cells from another untreated CLL patient (AllCells, California, USA) were resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.5×105 cells/well (30 μL/well) and 50 μL of a concentration series of IgG1-G28.1, IgG1-G28.1-E430G or IgG1-b12 was added (0.003-10 μg/mL final antibody concentration in 3.33×serial dilutions). .. After incubation (RT, 15 min), 20 μL of pooled normal human serum (NHS Cat #M0008 Sanquin, Amsterdam, The Netherlands) was added to each well as a source of complement and plates were incubated at 37° C. for 45 minutes.

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: The capacity to induce CDC was tested for a combination of bsIgG1-016-H5L2-LC90S-F405L-E430G×010-H5L2-K409R-E430G and an anti-CD20 antibody (IgG1-CD20-ofa; ofatumumab) on patient derived CLL tumor cells obtained from ConversantBio (Huntsville, Alabama, USA). .. Patient derived PBMCs were resuspended in RPMI containing 0.2% BSA (bovine serum albumin) and plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.1×10 6 cells/well (30 μL/well) and 50 μL of a concentration series of bsIgG1-016-H5L2-LC90S-F405L-E430G×010-H5L2-K409R-E430G (0.0625-0.05 μg/mL) and IgG1-CD20-ofa (1-8 μg/mL) was added in 2-fold dilutions. ..

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Raji cells, resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 1×105 cells/well (30 μL/well) and 50 μL of humanized CD37 antibodies, variants thereof, combinations thereof or control antibody IgG1-b12 was added (10 μg/mL final antibody concentration, combinations 5+5 μg/mL). .. After incubation (RT, 15 min, while shaking), 20 μL of pooled normal human serum (NHS Cat #M0008 Sanquin, Amsterdam, The Netherlands) was added to each well and plates were incubated at 37° C. for 45 minutes.

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Daudi cells, resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 1×10 5 cells/well (30 μL/well) and 50 μL of a concentration series of humanized CD37 antibodies and variants thereof, or control antibody IgG1-b12, was added (0.003-10 μg/mL final antibody concentration in 3.33×serial dilutions). .. After incubation (RT, 15 min), 20 μL of pooled normal human serum (NHS, Cat #M0008 Sanquin, Amsterdam, The Netherlands) was added to each well and plates were incubated at 37° C. for 45 minutes.

    FACS:

    Article Title: Bispecific and multispecific antibodies and method for isolation of such
    Article Snippet: .. For the FACS, cells (1×105 cells/well) were incubated in polystyrene 96-well round-bottom plates (Greiner bio-one 650101) with serial dilutions of antibody preparations (range 14 to 10000 ng/mL in 3-fold dilutions) in 100 μL PBS/0.1% BSA/0.02% azide at 4° C. for 30 min. After washing twice in PBS/0.1% BSA/0.02% azide, cells were incubated in 100 μL with secondary antibody at 4° C. for 30 min. As a secondary antibody, R-Phycoerythrin (PE)-conjugated goat-anti-human IgG F(ab′)2 (109-116-098, Jackson ImmunoResearch Laboratories, Inc.®, West Grove, PA) diluted 1/100 in PBS/0.1% BSA/0.02% azide, was used for all experiments. .. Next, cells were washed twice in PBS/0.1% BSA/0.02% azide, resuspended in 150 μL PBS/0.1% BSA/0.02% azide and analyzed on a FACS Canto IITM (BD Biosciences).

    Incubation:

    Article Title: Bispecific and multispecific antibodies and method for isolation of such
    Article Snippet: .. For the FACS, cells (1×105 cells/well) were incubated in polystyrene 96-well round-bottom plates (Greiner bio-one 650101) with serial dilutions of antibody preparations (range 14 to 10000 ng/mL in 3-fold dilutions) in 100 μL PBS/0.1% BSA/0.02% azide at 4° C. for 30 min. After washing twice in PBS/0.1% BSA/0.02% azide, cells were incubated in 100 μL with secondary antibody at 4° C. for 30 min. As a secondary antibody, R-Phycoerythrin (PE)-conjugated goat-anti-human IgG F(ab′)2 (109-116-098, Jackson ImmunoResearch Laboratories, Inc.®, West Grove, PA) diluted 1/100 in PBS/0.1% BSA/0.02% azide, was used for all experiments. .. Next, cells were washed twice in PBS/0.1% BSA/0.02% azide, resuspended in 150 μL PBS/0.1% BSA/0.02% azide and analyzed on a FACS Canto IITM (BD Biosciences).

    other:

    Article Title: Antibodies binding AXL
    Article Snippet: Cell Culture LS174T cells (human colorectal adenocarcinoma cell line; ATCC, cat no CL-188) were cultured in Minimum Essential Medium (MEM) with Glutamax, Hepes and Phenol Red (Life Technologies, cat no 42360-024).

    Control:

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Briefly, tumor cells derived from a CLL patient (as in first experiment described above), resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.5×105 cells/well (30 μL/well), centrifuged and 50 μL of CD37 (Abcam, cat. no. 76522) or control mouse antibody (Purified Mouse IgG1,κ Isotype Control, Clone MOPC-21; BD cat. no. 555746) was added. ..

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Raji cells, resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 1×105 cells/well (30 μL/well) and 50 μL of humanized CD37 antibodies, variants thereof, combinations thereof or control antibody IgG1-b12 was added (10 μg/mL final antibody concentration, combinations 5+5 μg/mL). .. After incubation (RT, 15 min, while shaking), 20 μL of pooled normal human serum (NHS Cat #M0008 Sanquin, Amsterdam, The Netherlands) was added to each well and plates were incubated at 37° C. for 45 minutes.

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Daudi cells, resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 1×10 5 cells/well (30 μL/well) and 50 μL of a concentration series of humanized CD37 antibodies and variants thereof, or control antibody IgG1-b12, was added (0.003-10 μg/mL final antibody concentration in 3.33×serial dilutions). .. After incubation (RT, 15 min), 20 μL of pooled normal human serum (NHS, Cat #M0008 Sanquin, Amsterdam, The Netherlands) was added to each well and plates were incubated at 37° C. for 45 minutes.

    Purification:

    Article Title: Methods of use of anti-CD37 antibodies, monoclonal anti-CD37 antibodies
    Article Snippet: .. Briefly, tumor cells derived from a CLL patient (as in first experiment described above), resuspended in RPMI containing 0.2% BSA, were plated into polystyrene 96-well round-bottom plates (Greiner bio-one Cat #650101) at a density of 0.5×105 cells/well (30 μL/well), centrifuged and 50 μL of CD37 (Abcam, cat. no. 76522) or control mouse antibody (Purified Mouse IgG1,κ Isotype Control, Clone MOPC-21; BD cat. no. 555746) was added. ..



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    polystyrene 96 well round bottom plates  (Greiner Bio)
    96
    Greiner Bio polystyrene 96 well round bottom plates
    Polystyrene 96 Well Round Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polystyrene+96+well+round+bottom+plates/Microplate+96+Well+Ps+U-Bottom+Clear/us12595316-357-9-13
    Average 96 stars, based on 1 article reviews
    polystyrene 96 well round bottom plates - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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